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HTS016M - ChemiSCREEN™ C3aR Anaphylotoxin Receptor Membrane Preparation

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$646.90
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HTS016M
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5 unit(s)
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  • Product Description

    C3a, along with C4a and C5a, is a 77 amino acid anaphylotoxin generated by proteolytic cleavage during activation of the complement pathway (Ember and Hugli, 1997). The anaphylotoxins strongly promote inflammation by recruiting leukocytes, particularly basophils, eosinophils, neutrophils and monocytes (Martin et al., 1997). The proinflammatory properties of C3a are mediated by interaction between the peptide and a 7-TM G-protein coupled receptor, C3aR (Crass et al., 1996). Genetic and pharmacological inhibition of C3a/C3aR interaction indicates an important role for C3aR in allergic asthma (Humbles et al., 2000; Drouin et al., 2002). C3a/C3aR also enhances the effect of SDF-1 in promoting retention of haematopoietic stem/progenitor cells within the bone marrow (Rataiczak et al., 2004). The C3aR membrane preps are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of C3aR. The membranes have been shown to bind to 125I-labeled C3a with a Kd of 0.15 nM. With 0.2 nM [125I]-C3a, 10 mg/well C3aR Membrane Prep yields greater than 5 fold signal-to-background ratio.

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    Item Unit of Measure: EA
    Quantity: 200 units
    Storage: On receipt of material store at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.
    Applications: Radioligand Binding Assay
    Species: Full-length human C3aR cDNA (Accession Number: U28488)
    Host Cell: Chem-1, an adherent cell line with no detectable endogenous C3aR expression.
    Reference 1: 1. Ember JA and Hugli TE (1997) Complement factors and their receptors. Immunopharmacology 38: 3-15.
    Reference 2: 2. Martin U, et al. (1997) The human C3a receptor is expressed on neutrophils and monocytes, but not on B or T lymphocytes. J. Exp. Med. 186: 199-207.
    Reference 3: 3. Crass T et al. (1996) Expression cloning of the human C3a anaphylatoxin receptor (C3aR) from differentiated U-937 cells. Eur. J. Immunol. 26: 1944-50.
    Reference 4: 4. Humbles AA et al. (2000) A role for the C3a anaphylatoxin receptor in the effector phase of asthma. Nature 406: 998-1001.
    Reference 5: 5. Drouin SM et al. (2002) Absence of the complement anaphylatoxin C3a receptor suppresses Th2 effector functions in a murine model of pulmonary allergy.J. Immunol. 169: 5926-33.
    Reference 6: 6. Ratajczak J et al. (2004) Mobilization studies in mice deficient in either C3 or C3a receptor (C3aR) reveal a novel role for complement in retention of hematopoietic stem/progenitor cells in bone marrow. Blood 103: 2071-8.
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